RESUMO
We compared 50 patients with head and neck squamous cell carcinomas (cases) and 45 matched healthy controls. Biopsy specimens were taken from tumours and normal tissue of the cases and controls respectively and serial paraffin embedded sections were processed to detect Epstein-Barr (EB) viral antigen. We found EB viral proteins in 38% of cases and none in controls, which suggests a positive correlation. Serum samples were also tested for the presence of EB virus IgG by ELISA for comparison with immunohistochemical findings. Patients with positive immunohistochemical staining results had significantly higher mean antibody titres compared with those with negative results. ELISA may be useful in determining the etiology of head and neck cancers, but the results are not unequivocally reliable.
Assuntos
Carcinoma de Células Escamosas/virologia , Infecções por Vírus Epstein-Barr/complicações , Neoplasias de Cabeça e Pescoço/virologia , Adulto , Distribuição por Idade , Anticorpos Antivirais/sangue , Biópsia , Carcinoma de Células Escamosas/patologia , Estudos de Casos e Controles , DNA Viral/análise , DNA Viral/genética , Egito/epidemiologia , Ensaio de Imunoadsorção Enzimática/normas , Infecções por Vírus Epstein-Barr/sangue , Infecções por Vírus Epstein-Barr/diagnóstico , Infecções por Vírus Epstein-Barr/epidemiologia , Feminino , Neoplasias de Cabeça e Pescoço/patologia , Herpesvirus Humano 4/genética , Herpesvirus Humano 4/imunologia , Humanos , Imunoglobulina G/sangue , Imuno-Histoquímica/normas , Masculino , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Sensibilidade e Especificidade , Distribuição por SexoRESUMO
We compared 50 patients with head and neck squamous cell carcinomas [cases] and 45 matched healthy controls. Biopsy specimens were taken from tumours and normal tissue of the cases and controls respectively and serial paraffin embedded sections were processed to detect Epstein-Barr [EB] viral antigen. We found EB viral proteins in 38% of cases and none in controls, which suggests a positive correlation. Serum samples were also tested for the presence of EB virus IgG by ELISA for comparison with immunohistochemical findings. Patients with positive immunohistochemical staining results had significantly higher mean antibody titres compared with those with negative results. ELISA may be useful in determining the etiology of head and neck cancers, but the results are not unequivocally reliable
Assuntos
Distribuição por Idade , Anticorpos Antivirais , Biópsia , Estudos de Casos e Controles , DNA Viral , Ensaio de Imunoadsorção Enzimática , Infecções por Vírus Epstein-Barr , Neoplasias de Cabeça e Pescoço , Imunoglobulina G , Estadiamento de Neoplasias , Carcinoma de Células EscamosasRESUMO
Hepatitis A virus (HAV) immunity in a group of socially and economically deficient preschool children in Alexandria has been investigated. A total of 228 randomly selected children were enrolled. Saliva was collected from every child using a cotton wool swab. Anti-HAV was investigated in the collected saliva by a competitive ELISA technique utilizing a commercially available kit. The results revealed that among the investigated children 60 (26.3%) were anti-HAV positive. The mean age of anti-HAV positive children was 4.43 years +/- 0.21 years. Ten (33.3%) couples were siblings, 2 (3.3%) children gave a positive history of jaundice. As regards housing conditions; 34 (56.7%) of anti-HAV positive children had indoor sources of water supply and 26 (43.3%) had outdoor ones. The mean crowding index was 3.35 +/- 1.72. Positive anti-HAV was significantly more prevalent among older (P < 0.05) and among female children (P < 0.02). In conclusion, the routine administration of hepatitis A vaccine, combined with improvements in sanitation should reduce the incidence of acute illness and complications associated with the disease thereby decreasing the spread of HAV in developing countries.
